Glutamate neurotoxicity in cortical cell culture
نویسندگان
چکیده
منابع مشابه
Glutamate neurotoxicity in cortical cell culture.
The central neurotoxicity of the excitatory amino acid neurotransmitter glutamate has been postulated to participate in the pathogenesis of the neuronal cell loss associated with several neurological disease states, but the complexity of the intact nervous system has impeded detailed analysis of the phenomenon. In the present study, glutamate neurotoxicity was studied with novel precision in di...
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We assayed glutamate transport activity in cultures of rat cortical neurons containing < 0.2% astrocytes. Using [3H]L-glutamate as the tracer, sodium-dependent high affinity glutamate transport was demonstrated [K(m) = 17.2 +/- 2.4 microM; Vmax = 3.3 +/- 0.32 nmol/mg of protein/min (n = 5)]. Dihydrokainate (1 mM) inhibited uptake of radioactivity by 88 +/- 3% and had a Ki value of 65 +/- 7 micr...
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ژورنال
عنوان ژورنال: The Journal of Neuroscience
سال: 1987
ISSN: 0270-6474,1529-2401
DOI: 10.1523/jneurosci.07-02-00357.1987